解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The most important rhizomania-resistance gene in sugar beet is the Rz1 gene from the Holly Sugar Company in California, the source widely used to breed partially resistant varieties. Other important gene sources are WB41 and WB42, which both originate from Beta vulgaris subsp. maritima collected in Denmark, and which ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-108
更新日期:2005-04-01 00:00:00
abstract::Fusarium head blight (FHB) is one of the most important fungal wheat diseases worldwide. Understanding the genetics of FHB resistance is the key to facilitating the introgression of different FHB resistance genes into adapted wheat. The objectives of the present study were to detect and map quantitative trait loci (QT...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-104
更新日期:2005-04-01 00:00:00
abstract::Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromos...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-087
更新日期:2005-02-01 00:00:00
abstract::The isolation, physical, and genetic mapping of a group of wheat genes expressed in infected heads of Triticum aestivum 'Frontana' resistant to Fusarium head blight is reported. A cDNA library was built from heads of 'Frontana' through suppressive subtractive hybridization, to enrich for sequences induced by the patho...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-098
更新日期:2005-02-01 00:00:00
abstract::We have used nuclear and chloroplast molecular markers to genotype cultivated and wild accessions of Vitis vinifera L. from Tunisia and assess their genetic relationships. Fifty-five distinct genotypes were identified among 80 cultivated accessions, including 18 genotypic groups containing between 2 and 5 accessions p...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-072
更新日期:2004-12-01 00:00:00
abstract::Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-073
更新日期:2004-12-01 00:00:00
abstract::The superoxide dismutases (SODs) protect oxygen-using cells against reactive oxygen species, the potentially toxic by-products of respiration, oxidative metabolism, and radiation. We have previously shown that genetic disruption of CuZn SOD (SOD1) in Drosophila imparts a recessive phenotype of reduced lifespan, infert...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-083
更新日期:2004-12-01 00:00:00
abstract::Disease lesion mimics provide an excellent biological system to study the genetic basis of cell death in plants. Many lesion mimics show variation in phenotype expression in different genetic backgrounds. Our goal was to identify quantitative trait loci (QTL) modifying lesion mimic expression thereby identifying genet...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-046
更新日期:2004-10-01 00:00:00
abstract::The genomic content of the subtelomeric repeated sequences Spelt1 and Spelt52 was studied by dot, Southern, and in situ hybridization in 11 newly synthesized amphiploids of Aegilops and Triticum, and data were compared with the parental plants. Spelt1 had reduced copy numbers in the first generation of three synthetic...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-044
更新日期:2004-10-01 00:00:00
abstract::A high-density BAC filter of Triticum monococcum was screened for the presence of a centromeric retrotransposon using the integrase region as a probe. Southern hybridization to the BAC digests using total genomic DNA probes of Triticum monococcum, Triticum aestivum, and Hordeum vulgare detected differentially hybridiz...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-034
更新日期:2004-08-01 00:00:00
abstract::Amplified fragment length polymorphism (AFLP) has proven a powerful tool for tagging genes or quantitative trait loci (QTLs) of interest in plants. However, conversion of AFLP markers into sequence-tagged site (STS) markers is technically challenging in wheat owing to the complicated nature of its genome. In this stud...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-022
更新日期:2004-08-01 00:00:00
abstract::Twelve genes encoding two closely related subtypes (ST1.1a and ST1.1b) of a sulphate transporter have been identified in the diploid wheats Aegilops tauschii, Triticum urartu, and Aegilops speltoides, as well as the hexaploid Triticum aestivum. Based on phylogenetic comparisons with other plant sulphate transporters, ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-011
更新日期:2004-06-01 00:00:00
abstract::We have developed 21 new microsatellites in the model diploid perennial species Fragaria vesca from an enriched genomic library developed using F. vesca 'Ruegen'. The transferability of the primer pairs to other Fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawber...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-142
更新日期:2004-06-01 00:00:00
abstract::Larvae of the black fly morphospecies Simulium vittatum from Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with 33P, and run on polyacrylamide gels. The entire da...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-144
更新日期:2004-04-01 00:00:00
abstract::TBP (tubulin-based polymorphism) is a new molecular marker based tool that relies on the presence of intron-specific DNA polymorphisms of the plant beta-tubulin gene family. The multifunctional and essential role of the tubulin proteins is reflected in the conservation of regions within their primary amino acid sequen...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-132
更新日期:2004-04-01 00:00:00
abstract::Cuscuta is a widely distributed genus of holoparasitic plants. Holocentric chromosomes have been reported only in species of one of its subgenera (Cuscuta subg. Cuscuta). In this work, a representative of this subgenus, Cuscuta approximata, was investigated looking for its mitotic and meiotic chromosome behaviour and ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-098
更新日期:2004-02-01 00:00:00
abstract::Wide hybrids have been used in generating genetic maps of many plant species. In this study, genetic and physical mapping was performed on ph1b-induced recombinants of rye chromosome 2R in wheat (Triticum aestivum L.). All recombinants were single breakpoint translocations. Recombination 2RS-2BS was absent from the te...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-089
更新日期:2004-02-01 00:00:00
abstract::Multiple-target fluorescence in situ hybridization (FISH) was applied on mitotic chromosomes of seven Lens taxa using two highly repetitive sequences (pLc30 and pLc7) isolated from the cultivated lentil and the multigene families for the 18S-5.8S-25S (pTa71) and 5S rRNA (pTa794) from wheat simultaneously as probes. Th...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-077
更新日期:2003-12-01 00:00:00
abstract::The ancestor of barley (Hordeum vulgare subsp. spontaneum) may be a source of novel alleles for crop improvement. We developed a set of recombinant chromosome substitution lines (RCSLs) using an accession of H. vulgare subsp. spontaneum (Caesarea 26-24, from Israel) as the donor and Hordeum vulgare subsp. vulgare 'Har...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-080
更新日期:2003-12-01 00:00:00
abstract::This short review presents a historical perspective of chromosome research during the last 50 years. It shows how molecular knowledge and technology of DNA entered cytogenetics step by step making it now daily practice in almost every modern chromosome lab. A crucial milestone in these decades has been the development...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/g03-107
更新日期:2003-12-01 00:00:00
abstract::Microsatellite markers are widely used in linkage mapping, parentage testing, population genetic studies, and molecular evolution studies in many agricultural species, while only a limited number of ostrich (Struthio camelus) microsatellites have been isolated. Thus, we constructed a random small-insert genomic librar...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-059
更新日期:2003-10-01 00:00:00
abstract::A major locus conferring resistance to the causal organism of powdery mildew, Erysiphe polygoni DC, in mungbean (Vigna radiata L. Wilczek) was identified using QTL analysis with a population of 147 recombinant inbred individuals. The population was derived from a cross between 'Berken', a highly susceptible variety, a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-057
更新日期:2003-10-01 00:00:00
abstract::An interspecific Gossypium hirsutum x Gossypium barbadense backcross population of 75 BC1 plants was evaluated for 1014 markers. The map consists of 888 loci, including 465 AFLPs, 229 SSRs, 192 RFLPs, and 2 morphological markers, ordered in 37 linkage groups that represent most if not all of the 26 chromosomes, altoge...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-050
更新日期:2003-08-01 00:00:00
abstract::Red clover is an important forage legume species for temperate regions and very little is known about the genetic organization of its breeding populations. We used random amplified polymorphic DNA (RAPD) genetic markers to address the genetic diversity and the distribution of variation in 20 breeding populations and c...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-030
更新日期:2003-08-01 00:00:00
abstract::For molecular and cytogenetic studies, two partial bacterial artificial chromosome (BAC) libraries of the garlic cultivar Allium sativum L. 'Danyang' were constructed using high molecular weight (HMW) garlic DNA, the pBAC1-SACB1 vector, and the pIndigoBAC536 vector. The average insert size of the BAC library was about...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-012
更新日期:2003-06-01 00:00:00
abstract::Bombyx mori L., commonly recognised around the world as the mulberry silkworm, is characterized by a wide variability in yield and developmental traits, which have been proven through conventional genetic analysis to be of polygenic nature. A large number of morpho-biochemical traits and RFLP and RAPD markers are mapp...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-024
更新日期:2003-06-01 00:00:00
abstract::Chamaecyparis nootkatensis is an ecologically and economically important conifer of the north Pacific coastal forests. To aid in studies of clonal structure and genetic differentiation of this and related species, we isolated and characterized microsatellites from C. nootkatensis. A microsatellite-enriched library yie...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-014
更新日期:2003-06-01 00:00:00
abstract::Abstract: Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-003
更新日期:2003-04-01 00:00:00
abstract::Forty apricot cultivars with different geographic origins belonging to the germplasm collections of St. Istvan University (Budapest, Hungary) and the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were studied by means of SSR markers. The aim of the study was to determine the genetic relatio...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-128
更新日期:2003-04-01 00:00:00
abstract::Asexual lineages in the parasitoid wasp Lysiphlebus fabarum (Hymenoptera: Braconidae: Aphidiinae) have previously been shown to have occasionally undergone sexual reproduction and recombination with males from related sexual populations. In the present study, the cytogenetic system of asexual females in this species i...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-112
更新日期:2003-02-01 00:00:00
abstract::Configurations of mitochondrial coxI and orfB gene regions were analysed by polymerase chain reaction (PCR) in three wild and one cultivated species of Raphanus. A total of 207 individual plants from 60 accessions were used. PCR with five combinations of primers identified five different amplification patterns both in...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-110
更新日期:2003-02-01 00:00:00
abstract::Five wheat-barley translocations in a wheat background were characterized through the combination of cytogenetic and molecular genetic approaches. The wheat chromosome segments involved in the translocations were identified using sequential GISH and two-colour FISH with the probes pSc119.2 and pAs1. The barley chromat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-068
更新日期:2002-12-01 00:00:00
abstract::The use of consensus chloroplast microsatellites primers for dicotyledonous chloroplast genomes revealed the existence of intra and interspecific length variation within the genus Vitis. Three chloroplast microsatellite loci were found to be polymorphic in samples of Vitis vinifera, Vitis berlandieri, Vitis riparia, a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-087
更新日期:2002-12-01 00:00:00
abstract::Orobanche crenata Forsk. is a root parasite that produces devastating effects on many crop legumes and has become a limiting factor for faba bean production in the Mediterranean region. The efficacy of available control methods is minimal and breeding for broomrape resistance remains the most promising method of contr...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-082
更新日期:2002-12-01 00:00:00
abstract::Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-049
更新日期:2002-10-01 00:00:00
abstract::In multi-cellular organisms, failure to properly regulate cell-cycle progression can result in inappropriate cell death or uncontrolled cell division leading to tumor formation. To guard against such events, conserved regulatory mechanisms called "checkpoints" block progression into mitosis in response to DNA damage a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-051
更新日期:2002-10-01 00:00:00
abstract::Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross b...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-043
更新日期:2002-10-01 00:00:00
abstract::B chromosome derivatives suffering from breaks within their centromere were examined cytologically and molecularly. We showed by high resolution FISH that misdivision of the centromere of a univalent chromosome can occur during meiosis. The breaks divide the centromere repeat sequence cluster. A telocentric chromosome...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-023
更新日期:2002-08-01 00:00:00
abstract::A single cross between two clones of passion fruit (Passiflora edulis Sims. f. flavicarpa Deg., 2n = 18) was selected for genetic mapping. The mapping population was composed of 90 F1 plants derived from a cross between 'IAPAR 123' (female parent) and 'IAPAR 06' (male parent). A total of 380 RAPD primers were analyzed...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-035
更新日期:2002-08-01 00:00:00
abstract::The SRY gene is a single-copy, male-specific gene, located on the Y chromosome in most mammals. However, recently we have described the presence of multiple polymorphic copies of this gene in both males and females of the vole species Microtus cabrerae. Here, we present the chromosomal localization of SRY gene copies ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-007
更新日期:2002-06-01 00:00:00